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Fully Active Insulin by Selective Formation of the Disulfide Bridges between a Sythetic A‐Chain and Natural B‐Chain
Author(s) -
Birr Christian,
Pipkorn Rüdiger
Publication year - 1979
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.197905362
Subject(s) - disulfide bond , chain (unit) , insulin , chemistry , yield (engineering) , sulfur , combinatorial chemistry , stereochemistry , biochemistry , organic chemistry , materials science , biology , endocrinology , physics , astronomy , metallurgy
Directed formation of the three disulfide bridges in insulin is a decisive synthetic step. Only 10% of insulin is formed on random recombination of A‐ and B‐ chains (1:1). An A‐chain synthesized with selectively removable sulfur protecting groups on a polymeric carrier was allowed to react with a natural B‐chain. The yield of fully active insulin increases to 25%.