Premium
Restriction Endonucleases
Author(s) -
Arber Werner
Publication year - 1978
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.197800733
Subject(s) - restriction enzyme , recognition sequence , dna , biology , endonuclease , genetics , isoschizomer , computational biology , dna methylation , gene , gene expression
Abstract Restriction endonucleases are enzymes commonly found in bacteria which can fragment DNA. Cleavage of the DNA molecule takes place after recognition of a nucleotide sequence which is specific for each restriction endonuclease. Some of the enzymes split the DNA within the recognition sequence. This property allows these enzymes to be used for the systematic fragmentation of DNA molecules in structural and functional analyses of genetic material. The biological function of restriction endonucleases could be to provide a sort of primitive immune system which enables the cells to destroy foreign genetic material. Methylation of nucleotides lying within the recognition sequence serves to protect the cell's own DNA from being degraded by its restriction endonucleases. Restriction endonucleases were discovered during the investigation of the restriction of viral growth observed on changing hosts.