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Investigation of Enzyme Mechanisms with Nucleoside Phosphorothioates
Author(s) -
Eckstein Fritz
Publication year - 1975
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.197501601
Subject(s) - chemistry , nucleoside , thiophosphate , diastereomer , enzyme , nucleotide , stereochemistry , substrate (aquarium) , nucleoside triphosphate , sulfur , deoxyadenosine , nucleobase , organic chemistry , biochemistry , dna , biology , ecology , gene
Nucleoside phosphorothioates are modified nucleotides in which an oxygen atom bound to a phosphorus atom has been replaced by a sulfur atom. These compounds are suitable for the investigation of enzyme mechanisms because, although they are bound by enzymes in many cases just as well as the natural substrate, the enzymatic reactions very often proceed considerably more slowly. Since nucleoside thiophosphate O ‐esters exist as diastereomers these compounds can be used in suitable cases for information about the stereochemistry of enzymatic reactions.