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Bifunctional Reagents for the Crosslinking of Proteins
Author(s) -
Fasold Hugo,
Klappenberger Jürgen,
Meyer Christa,
Remold Heinz
Publication year - 1971
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.197107951
Subject(s) - bifunctional , reagent , amino acid , protein quaternary structure , chemistry , side chain , bridge (graph theory) , amino acid residue , salt bridge , combinatorial chemistry , stereochemistry , biochemistry , peptide sequence , organic chemistry , biology , polymer , catalysis , anatomy , protein subunit , mutant , gene
Proteins can be crosslinked by chemical compounds without any accompanying change in the complicated three‐dimensional structure. Many bifunctional reagents that can react with functional groups in the side chains of the amino acids are now known. Not only can the distance between the linked amino acid residues be deduced from the known length of the bridge introduced, but information can also be obtained concerning changes in conformation during other reactions and concerning the arrangement of the components of a quaternary structure.