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The Anomalous Temperature Dependence of Enzyme‐Catalyzed Reactions
Author(s) -
Talsky Gerhard
Publication year - 1971
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.197105481
Subject(s) - substrate (aquarium) , ionic strength , chemistry , catalysis , active center , reaction rate , active site , enzyme , molecule , thermodynamics , physics , organic chemistry , biology , aqueous solution , ecology
Breaks or curvatures in the plot of log v br vs. 1/T have been described in the literature for a number of reactions. If sufficiently small temperature intervals are used in the investigation of the temperature dependence (STI method) a trend line is obtained instead. It is then possible to distinguish regions of linear behavior and regions of anomalous behavior. The effects of e. g. the pH, the ionic strength, and added salts as well as of the size of the enzyme and substrate molecules on the anomalies were examined. The anomalous temperature dependence of the reaction rate was found to be a particularly sensitive indicator of the reaction event on the enzyme. The course of the reaction is evidently affected even by relatively small changes in conformation, particularly in the region of the active center.