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Automatic Analysis of Amino‐Acid Sequences in Proteins and Peptides
Author(s) -
von. Wilm M.
Publication year - 1970
Publication title -
angewandte chemie international edition in english
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 0570-0833
DOI - 10.1002/anie.197002671
Subject(s) - myoglobin , centrifugation , amino acid analysis , amino acid , edman degradation , chromatography , chemistry , sequence (biology) , extraction (chemistry) , peptide sequence , biochemistry , computer science , gene
Abstract The development of the widely used automatic method from Edman's manual sequence analysis of proteins and peptides has resulted from modification of the procedure and from the use of a physically novel principle. All reaction, centrifugation, extraction, and drying operations are carried out in a rotating vessel. At a minimal cost in time and material, for example, sixty amino acids have been split off consecutively from a single sample of myoglobin.