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A Cyanine‐Mediated Self‐Assembly System for the Construction of a Two‐in‐One Nanodrug
Author(s) -
Wang Haihui,
Xiao Han,
Zhu Xiaohui,
Liu Yongfei,
Fu Zhangcheng,
Li Chunsen,
Lu Chunhua,
Yang Huanghao
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202108393
Subject(s) - dna , cyanine , nucleic acid , oligonucleotide , chemistry , drug , molecule , combinatorial chemistry , nanotechnology , biophysics , doxorubicin , materials science , biochemistry , organic chemistry , biology , pharmacology , fluorescence , physics , genetics , chemotherapy , quantum mechanics
Abstract The combination of gene therapy and chemotherapy provides a We developed a simple and versatile approach to prepare a series of two‐in‐one nanodrugs through direct self‐assembly of cyanine‐labeled single‐stranded DNA (Cys‐DNA) and different types of drug molecules. Molecular dynamics simulation showed that the Cys introduced into the DNA could enhance the noncovalent interaction between Cys‐DNA and drug molecules. More drug molecules were incorporated into Cys‐DNA, tending to spontaneously form hybrid Cys‐DNA/drug nanosphere. Such nanospheres serve as both carriers and cargoes, excluding the extra use of nontherapeutic excipients and showing ultrahigh drug loading capacity. Following this approach, an antisense oligonucleotides/doxorubicin nanodrug model was constructed, demonstrating the significant synergistic anti‐tumor therapeutic effect. As a proof of the concept, our study establishes a simple and reproducible two‐in‐one nucleic acid‐based drug formulation.