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Precise Encoding of Triple‐Bond Raman Scattering of Single Polymer Nanoparticles for Multiplexed Imaging Application
Author(s) -
Zhu Wei,
Cai ErLi,
Li HaoZheng,
Wang Ping,
Shen AiGuo,
Popp Jürgen,
Hu JiMing
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202106136
Subject(s) - monomer , raman scattering , polymerization , triple bond , nanoparticle , polymer , raman spectroscopy , materials science , nanotechnology , molecular recognition , chemistry , molecule , optics , polymer chemistry , double bond , organic chemistry , physics , composite material
Stimulated Raman scattering (SRS) microscopy in combination with innovative tagging strategies offers great potential as a universal high‐throughput biomedical imaging tool. Here, we report rationally tailored small molecular monomers containing triple‐bond units with large Raman scattering cross‐sections, which can be polymerized at the nanoscale for enhancement of SRS contrast with smaller but brighter optical nanotags with artificial fingerprint output. From this, a class of triple‐bond rich polymer nanoparticles (NPs) was engineered by regulating the relative dosages of three chemically different triple‐bond monomers in co‐polymerization. The bonding strategy allowed for 15 spectrally distinguishable triple‐bond combinations. These accurately structured nano molecular aggregates, rather than long‐chain macromolecules, could establish a universal method for generating small‐sized biological SRS imaging tags with high sensitivity for high‐throughput multi‐color biomedical imaging.