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Supramolecular Bioimaging through Signal Amplification by Combining Indicator Displacement Assay with Förster Resonance Energy Transfer
Author(s) -
Geng WenChao,
Ye Zhongju,
Zheng Zhe,
Gao Jie,
Li JuanJuan,
Shah Muhammad Raza,
Xiao Lehui,
Guo DongSheng
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202104358
Subject(s) - förster resonance energy transfer , supramolecular chemistry , nanotechnology , covalent bond , fluorescence , chemistry , materials science , physics , molecule , organic chemistry , quantum mechanics
Fluorescent chemosensors are powerful imaging tools in the fields of life sciences and engineering. Based on the principle of supramolecular chemistry, indicator displacement assay (IDA) provides an alternative approach for constructing and optimizing chemosensors, which has the advantages of simplicity, tunability, and modularity. However, the application of IDA in bioimaging continues to face a series of challenges, including interfering signals, background noise, and inconsistent spatial location. Accordingly, we herein report a supramolecular bioimaging strategy of Förster resonance energy transfer (FRET)‐assisted IDA by employing macrocyclic amphiphiles as the operating platform. By merging FRET with IDA, the limitations of IDA in bioimaging were addressed. As a proof of concept, the study achieved mitochondria‐targeted imaging of adenosine triphosphate in live cells with signal amplification. This study opens a non‐covalent avenue for bioimaging with advancements in tunability, generality, and simplicity, apart from the covalent approach.