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Real‐Time NMR Monitoring of Spatially Segregated Enzymatic Reactions in Multilayered Hydrogel Assemblies **
Author(s) -
Nordin Nurdiana,
Bordonali Lorenzo,
Davoodi Hossein,
Ratnawati Novindi Dwi,
Gygli Gudrun,
Korvink Jan G.,
Badilita Vlad,
MacKin Neil
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202103585
Subject(s) - microscale chemistry , glucose oxidase , reagent , chemistry , nanotechnology , chitosan , self healing hydrogels , chemical engineering , combinatorial chemistry , materials science , enzyme , organic chemistry , mathematics education , mathematics , engineering
Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high‐resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi‐layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real‐time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.