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Bispecific Aptamer Chimeras Enable Targeted Protein Degradation on Cell Membranes
Author(s) -
Miao Yanyan,
Gao Qianqian,
Mao Menghan,
Zhang Chao,
Yang Liqun,
Yang Yang,
Han Da
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202102170
Subject(s) - aptamer , membrane protein , microbiology and biotechnology , chemistry , membrane , protein degradation , lysosome , biochemistry , biology , enzyme
The ability to regulate membrane protein abundance offers great opportunities for developing therapeutic sites for various diseases. Herein, we describe a platform for the targeted degradation of membrane‐associated proteins using bispecific aptamer chimeras that bind both the cell‐surface lysosome‐shuttling receptor (IGFIIR) and the targeted membrane‐bound proteins of interest. We demonstrate that the aptamer chimeras can efficiently and quickly shuttle the therapeutically relevant membrane proteins of Met and PTK‐7 to lysosomes and degrade them through the lysosomal protein degradation machinery. We anticipate that our method will provide a universal platform for the use of readily synthesized aptamer materials for biochemical research and potential therapeutics.