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pH‐Amplified CRET Nanoparticles for In Vivo Imaging of Tumor Metastatic Lymph Nodes
Author(s) -
Wang Zenghui,
Xia Heming,
Chen Binlong,
Wang Yaoqi,
Yin Qingqing,
Yan Yue,
Yang Ye,
Tang Mingmei,
Liu Jianxiong,
Zhao Ruiyang,
Li Wenzhe,
Zhang Qiang,
Wang Yiguang
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202102044
Subject(s) - lymph , in vivo , preclinical imaging , chemistry , metastasis , pathology , ex vivo , cancer research , biomedical engineering , cancer , medicine , biology , microbiology and biotechnology
Abstract Noninvasive imaging strategies have been extensively investigated for in vivo mapping of sentinel lymph nodes (SLNs). However, the current imaging strategies fail to accurately assess tumor metastatic status in SLNs with high sensitivity. Here we report pH‐amplified self‐illuminating near‐infrared nanoparticles, which integrate chemiluminescence resonance energy transfer (CRET) and signal amplification strategy, enabling accurate identification of metastatic SLNs. After draining into lymph nodes, the nanoparticles were phagocytosed and dissociated in acidic phagosomes of inflammatory macrophages to emit near‐infrared luminescent light. Using these nanoparticles, we successfully differentiated tumor metastatic lymph nodes from benign ones. These nanoparticles also exhibited excellent imaging capability for early detection of metastatic SLNs in diverse animal tumor models with small tumor volume (100–200 mm 3 ).