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Synthesis, Characterization, and Nanodisc Formation of Non‐ionic Polymers **
Author(s) -
Ravula Thirupathi,
Ramamoorthy Ayyalusamy
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202101950
Subject(s) - nanodisc , chemistry , polymer , amphiphile , surface modification , ionic liquid , ionic bonding , divalent , membrane , drug delivery , nanotechnology , combinatorial chemistry , lipid bilayer , biophysics , materials science , organic chemistry , ion , biochemistry , copolymer , catalysis , biology
Abstract Although lipid nanodiscs are increasingly used in the structural studies of membrane proteins, drug delivery and other applications, the interaction between the nanodisc belt and the protein to be reconstituted is a major limitation. To overcome this limitation and to further broaden the scope of nanodiscs, a family of non‐ionic amphiphilic polymers synthesized by hydrophobic functionalization of fructo‐oligosaccharides/inulin is reported. We show the stability of lipid nanodiscs formed by these polymers against pH and divalent metal ions, and their magnetic‐alignment properties. The reported results also demonstrate that the non‐ionic polymers extract membrane proteins with unprecedented efficiency.