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Rational Design of a Highly Selective Near‐Infrared Two‐Photon Fluorogenic Probe for Imaging Orthotopic Hepatocellular Carcinoma Chemotherapy
Author(s) -
Wu Xiaofeng,
Wang Rui,
Qi Sujie,
Kwon Nahyun,
Han Jingjing,
Kim Heejeong,
Li Haidong,
Yu Fabiao,
Yoon Juyoung
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202101190
Subject(s) - in vivo , hepatocellular carcinoma , molecular imaging , in situ , fluorescence lifetime imaging microscopy , preclinical imaging , chemotherapy , fluorescence , two photon excitation microscopy , cancer research , pathology , chemistry , medicine , biology , optics , physics , microbiology and biotechnology , organic chemistry
Selective fluorescence imaging of biomarkers in vivo and in situ for evaluating orthotopic hepatocellular carcinoma (HCC) chemotherapy remains a great challenge due to current imaging agents suffering from the potential interferences of other hydrolases. Herein, we observed that carbamate unit showed a high selectivity toward the HCC‐related biomarker carboxylesterase (CE) for evaluation of treatment. A near‐infrared two‐photon fluorescent probe was developed to not only specially image CE activity in vivo and in situ but also target orthotopic liver tumor after systemic administration. The in vivo signals of the probe correlate well with tumor apoptosis, making it possible to evaluate the status of treatment. The probe enables the imaging of CE activity in situ with a high‐resolution three‐dimensional view for the first time. This study may promote advances in optical imaging approaches for precise imaging‐guided diagnosis of HCC in situ and its evaluation of treatment.