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Effects of Sequence and Base Composition on the CD and TDS Profiles of i‐DNA
Author(s) -
Iaccarino Nunzia,
Cheng Mingpan,
Qiu Dehui,
Pagano Bruno,
Amato Jussara,
Di Porzio Anna,
Zhou Jun,
Randazzo Antonio,
Mergny JeanLouis
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202016822
Subject(s) - cytosine , dna , circular dichroism , chemistry , base pair , dna sequencing , absorbance , protonation , crystallography , stereochemistry , biochemistry , organic chemistry , chromatography , ion
The i‐motif DNA, also known as i‐DNA, is a non‐canonical DNA secondary structure formed by cytosine‐rich sequences, consisting of two intercalated parallel‐stranded duplexes held together by hemi‐protonated cytosine–cytosine + (C:C + ) base pairs. The growing interest in the i‐DNA structure as a target in anticancer therapy increases the need for tools for a rapid and meaningful interpretation of the spectroscopic data of i‐DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV‐absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i‐DNA‐forming sequences.