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Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs
Author(s) -
Novakovic Mihajlo,
Kupče Ēriks,
Scherf Tali,
Oxenfarth Andreas,
Schnieders Robbin,
Grün J. Tassilo,
WirmerBartoschek Julia,
Richter Christian,
Schwalbe Harald,
Frydman Lucio
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202015948
Subject(s) - two dimensional nuclear magnetic resonance spectroscopy , chemistry , magnetization transfer , nucleic acid , nuclear magnetic resonance spectroscopy , rna , magnetization , sensitivity (control systems) , nuclear overhauser effect , chemical physics , stereochemistry , physics , biochemistry , medicine , quantum mechanics , electronic engineering , gene , magnetic field , magnetic resonance imaging , engineering , radiology
2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent‐driven exchanges to enhance NOE correlations between exchangeable and non‐exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross‐peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS‐CoV‐2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.