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Probing the Intracellular Dynamics of Nitric Oxide and Hydrogen Sulfide Using an Activatable NIR II Fluorescence Reporter
Author(s) -
Zhu Tianli,
Ren Ning,
Liu Xia,
Dong Yan,
Wang Rongchen,
Gao Jinzhu,
Sun Jie,
Zhu Ying,
Wang Lihua,
Fan Chunhai,
Tian He,
Li Jiang,
Zhao Chunchang
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202015650
Subject(s) - fluorescence , chemistry , intracellular , hydrogen sulfide , biophysics , nitric oxide , biochemistry , organic chemistry , biology , sulfur , physics , quantum mechanics
Understanding the complex interplay among gasotransmitters is of great significance but remains technically challenging. In this study, we present the design and synthesis of a dually responsive BOD‐NH‐SC reporter for probing the dynamic and alternating existence of NO and H 2 S in living cells. This designed reporter can repeatedly cycle S‐nitrosation and transnitrosation reactions when successively treated with NO and H 2 S, thus affording the interchange of NIR fluorescence at 645 nm (NO) and NIR II fluorescence at 936 nm (H 2 S). In light of this unique fluorescence alternation between two colors, we synthesized water‐soluble BOD‐NH‐SC dots to visualize the intracellular dynamics of NO and H 2 S. These molecular probes thus provide a toolbox to elucidate the interplaying roles of NO and H 2 S in the complex interaction networks of various signal transduction pathways.