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High‐Preservation Single‐Cell Operation through a Photo‐responsive Hydrogel‐Nanopipette System
Author(s) -
Li ZiYuan,
Liu YingYa,
Li YuanJie,
Wang Wenhui,
Song Yanyan,
Zhang Junji,
Tian He
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202013011
Subject(s) - cell , drug delivery , nanotechnology , cell survival , intracellular , cell damage , viability assay , single cell analysis , materials science , chemistry , biophysics , computer science , biology , in vitro , biochemistry
Abstract Single‐cell and in situ cell‐based operation with nanopipette approach offers a possibility to elucidate the intracellular processes and may aid the improvement of therapy efficiency and precision. We present here a photo‐responsive hydrogel‐nanopipette hybrid system that can achieve single‐cell operation with high spatial/temporal resolution and negligible cell damage. This strategy overcomes long‐time obstacles in nanopipette single‐cell studies as high electric potential (ca. 1000 mV) or organic solvent is always used during operations, which would inevitably impose disturbance and damage to targeted cells. The light‐triggered system promotes a potential‐free, non‐invasive single‐cell injection, resulting in a well‐retained cell viability (90 % survival rate). Moreover, the photo‐driven injection enables a precisely dose‐controllable single‐cell drug delivery. Significantly reduced lethal doses of doxorubicin (163–217 fg cell −1 ) are demonstrated in corresponding cell lines.