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An Integrated Cofactor/Co‐Product Recycling Cascade for the Biosynthesis of Nylon Monomers from Cycloalkylamines
Author(s) -
Sarak Sharad,
Sung Sihyong,
Jeon Hyunwoo,
Patil Mahesh D.,
Khobragade Taresh P.,
Pagar Amol D.,
Dawson Philip E.,
Yun Hyungdon
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202012658
Subject(s) - cascade , cascade reaction , chemistry , combinatorial chemistry , cofactor , biosynthesis , biocatalysis , monomer , caprolactam , amino acid , natural product , organic chemistry , enzyme , polymer , catalysis , biochemistry , chromatography , reaction mechanism
We report a highly atom‐efficient integrated cofactor/co‐product recycling cascade employing cycloalkylamines as multifaceted starting materials for the synthesis of nylon building blocks. Reactions using E. coli whole cells as well as purified enzymes produced excellent conversions ranging from >80 and 95 % into desired ω‐amino acids, respectively with varying substrate concentrations. The applicability of this tandem biocatalytic cascade was demonstrated to produce the corresponding lactams by employing engineered biocatalysts. For instance, ϵ‐caprolactam, a valuable polymer building block was synthesized with 75 % conversion from 10 mM cyclohexylamine by employing whole‐cell biocatalysts. This cascade could be an alternative for bio‐based production of ω‐amino acids and corresponding lactam compounds.