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Bright and Stable NIR‐II J‐Aggregated AIE Dibodipy‐Based Fluorescent Probe for Dynamic In Vivo Bioimaging
Author(s) -
Zhang Qisong,
Yu Peng,
Fan Yong,
Sun Caixia,
He Haisheng,
Liu Xuan,
Lu Lingfei,
Zhao Mengyao,
Zhang Hongxin,
Zhang Fan
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202012427
Subject(s) - fluorescence , near infrared spectroscopy , absorption (acoustics) , in vivo , chemistry , aqueous solution , materials science , molar absorptivity , quantum yield , fluorescence lifetime imaging microscopy , optics , physics , microbiology and biotechnology , composite material , biology
Organic dyes emitting in the second near‐infrared (NIR‐II, 900–1700 nm) window, with high molar extinction coefficients (MEC) and quantum yields (QY) in aqueous, are essential for in vivo bioimaging and biosensing. In this work, we developed a dibodipy‐based aggregation‐induced emission (AIE) fluorescent probe, THPP , to meet this aim. THPP exhibits a high MEC and has intensified absorption and emission in J‐aggregated state, which significantly enhance the fluorescence intensity (≈55 folds) and extend the maximal absorption/emission wavelengths to 970/1010 nm in NIR‐II region. Based on the bright THPP , imaging with a high frame rate (34 frames per second) at a deep “valid penetration depth” up to 6 mm can be achieved. This enabled simultaneous and dynamic imaging of vasculatures and deep tissues. Besides, we succeeded in monitoring the respiratory rate of acute‐lung‐injury mice and tracing the collateral circulation process with a high frame rate.