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Interaction of α‐Synuclein with Phospholipids and the Associated Restructuring of Interfacial Lipid Water: An Interface‐Selective Vibrational Spectroscopic Study
Author(s) -
Biswas Biswajit,
Roy Subhadip,
Mondal Jahur Alam,
Singh Prashant Chandra
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202011179
Subject(s) - chemistry , monolayer , membrane , lipid bilayer , chemical physics , membrane lipids , biophysics , chemical engineering , crystallography , biochemistry , engineering , biology
Interaction of α‐Synuclein (αS) with biological lipids is crucial for the onset of its fibrillation at the cell membrane/water interface. Probed herein is the interaction of αS with membrane‐mimicking lipid monolayer/water interfaces. The results depict that αS interacts negligibly with zwitterionic lipids, but strongly affects the pristine air/water and charged lipid/water interfaces by perturbing the structure and orientation of the interfacial water. The net negative αS (−9 in bulk water; pH 7.4) reorients the water as hydrogen‐up (H‐up) at the air/water interface, and electrostatically interacts with positively charged lipids, making the interface nearly net neutral. αS also interacts with negatively charged lipids: the net H‐up orientation of the interfacial water decreases at the anionic lipid/water interface, revealing a domain‐specific interaction of net negative αS with the negatively charged lipids at the membrane surface.