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Engineering Micrometer‐Sized DNA Tracks for High‐Speed DNA Synthesis and Biosensing
Author(s) -
Wang Liying,
Song Kaiyun,
Qu Yuanyuan,
Chang Yangyang,
Li Zhongping,
Dong Chuan,
Liu Meng,
Brennan John D.,
Li Yingfu
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202010693
Subject(s) - dna , primer (cosmetics) , rolling circle replication , dna polymerase , dna nanotechnology , sequence (biology) , a dna , nanotechnology , computational biology , chemistry , biology , genetics , materials science , organic chemistry
φ 29 DNA polymerase (Pol φ 29) is capable of synthesizing long‐chain single‐stranded (ss) DNA molecules by copying the sequence of a small ss circular DNA template (ssCDT) in a process known as rolling circle amplification (RCA). The use of a ssCDT in RCA, however, comes with a key drawback: the rate of DNA synthesis is significantly reduced. We hypothesize that this issue can be overcome using a very long linear ssDNA template with a repeating sequence. To test this idea, we engineered a DNA assembly, which we denote “micrometer‐sized DNA track” (μDT). This μDT, with an average length of ≈13.5 μm, is made of a long chain DNA with a primer‐binding domain at its 3′ end and ≈1000 repeating sequence units at its 5′ end, each carrying a DNA anchor. We find that Pol φ 29 copies μDT at a speed ≈5‐time faster than it does a related ssCDT. We use this to design a simple all‐in‐one printed paper device for rapid and sensitive detection of microRNA let‐7. This paper sensor is capable of detecting 1 pM let‐7a in 10 minutes.