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Hyperfluorescence Imaging of Kidney Cancer Enabled by Renal Secretion Pathway Dependent Efflux Transport
Author(s) -
Du Bujie,
Chong Yue,
Jiang Xingya,
Yu Mengxiao,
Lo UGling,
Dang Andrew,
Chen YuAn,
Li Siqing,
Hernandez Elizabeth,
Lin Jason C.,
Hsieh JerTsong,
Zheng Jie
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202010187
Subject(s) - efflux , kidney , indocyanine green , chemistry , secretion , p glycoprotein , transporter , fluorophore , renal physiology , cancer research , biochemistry , microbiology and biotechnology , medicine , pathology , biology , fluorescence , multiple drug resistance , physics , quantum mechanics , gene , antibiotics
Abstract Renal tubular secretion is an active efflux pathway for the kidneys to remove molecules but has yet to be used to enhance kidney cancer targeting. We report indocyanine green (ICG) conjugated with a 2100 Da PEG molecule (ICG‐PEG45) as a renal‐tubule‐secreted near‐infrared‐emitting fluorophore for hyperfluorescence imaging of kidney cancers, which cannot be achieved with hepatobiliary‐ and glomerular‐clearable ICG. This pathway‐dependent targeting of kidney cancer arises from the fact that the secretion pathway enables ICG‐PEG45 to be effectively effluxed out of normal proximal tubules through P‐glycoprotein transporter while being retained in cancerous kidney tissues with low P‐glycoprotein expression. Tuning elimination pathways and utilizing different efflux kinetics of medical agents in normal and diseased tissues could be a new strategy for tackling challenges in disease diagnosis and treatments that cannot be addressed with passive and ligand‐receptor‐mediated active targeting.