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A Genetically Encoded RNA Photosensitizer for Targeted Cell Regulation
Author(s) -
Ren Kewei,
Keshri Puspam,
Wu Rigumula,
Sun Zhining,
Yu Qikun,
Tian Qian,
Zhao Bin,
Bagheri Yousef,
Xie Yiwen,
You Mingxu
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202010106
Subject(s) - photosensitizer , optogenetics , rna , aptamer , chemistry , biophysics , computational biology , biology , microbiology and biotechnology , nanotechnology , biochemistry , materials science , gene , photochemistry , neuroscience
Genetically encoded RNA devices have emerged for various cellular applications in imaging and biosensing, but their functions as precise regulators in living systems are still limited. Inspired by protein photosensitizers, we propose here a genetically encoded RNA aptamer based photosensitizer (GRAP). Upon illumination, the RNA photosensitizer can controllably generate reactive oxygen species for targeted cell regulation. The GRAP system can be selectively activated by endogenous stimuli and light of different wavelengths. Compared with their protein analogues, GRAP is highly programmable and exhibits reduced off‐target effects. These results indicate that GRAP enables efficient noninvasive target cell ablation with high temporal and spatial precision. This new RNA regulator system will be widely used for optogenetics, targeted cell ablation, subcellular manipulation, and imaging.