Premium
A General Strategy for Development of Activatable NIR‐II Fluorescent Probes for In Vivo High‐Contrast Bioimaging
Author(s) -
Ren TianBing,
Wang ZhiYao,
Xiang Zhen,
Lu Peng,
Lai HuanHua,
Yuan Lin,
Zhang XiaoBing,
Tan Weihong
Publication year - 2021
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202009986
Subject(s) - fluorescence , in vivo , chemistry , quantum yield , stokes shift , aggregation induced emission , combinatorial chemistry , preclinical imaging , quenching (fluorescence) , autofluorescence , biophysics , nanotechnology , photochemistry , materials science , physics , microbiology and biotechnology , biology , quantum mechanics
Abstract Organic dye based NIR‐II fluorescent probes, owing to their high signal‐to‐background ratio and deeper penetration, are highly useful for deep‐tissue high‐contrast imaging in vivo. However, it is still a challenge to design activatable NIR‐II fluorescent probes. Here, a novel class of polymethine dyes (NIRII‐RTs), with bright (quantum yield up to 2.03 %), stable, and anti‐solvent quenching NIR‐II emission, together with large Stokes shifts, was designed. Significantly, the novel NIR‐II dyes NIRII‐RT3 and NIRII‐RT4, equipped with a carboxylic acid group, can serve as effective NIR‐II platforms for the design of activatable bioimaging probes with high contrast. As a proof of concept, a series of target‐activatable NIRII‐RT probes (NIRII‐RT‐pH, NIRII‐RT‐ATP and NIRII‐RT‐Hg) for pH, adenosine triphosphate (ATP), and metal‐ion detection, were synthesized. By applying the NIRII‐RT probe, the real‐time monitoring of drug‐induced hepatotoxicity was realized.