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A Generalizable and Noncovalent Strategy for Interfacing Aptamers with a Microelectrode for the Selective Sensing of Neurotransmitters In Vivo
Author(s) -
Hou Hanfeng,
Jin Ying,
Wei Huan,
Ji Wenliang,
Xue Yifei,
Hu Jingbo,
Zhang Meining,
Jiang Ying,
Mao Lanqun
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202008284
Subject(s) - aptamer , interfacing , neurochemical , nanotechnology , alkyl , microelectrode , amphiphile , in vivo , chemistry , biosensor , biophysics , combinatorial chemistry , neuroscience , materials science , computer science , biology , organic chemistry , electrode , genetics , microbiology and biotechnology , computer hardware , copolymer , polymer
The selective sensing of neurochemicals is essential for understanding the chemical basis of brain function and pathology. Interfacing the excellent recognition features of aptamers with in vivo compatible carbon fiber microelectrode (CFE)‐based electroanalytical systems offers a plausible means to achieve this end. However, this is challenging in terms of coupling chemistry, stability, and versatility. Here, we present a new interfacial functionalization strategy based on the assembly of aptamer cholesterol amphiphiles (aptCAs) on the alkyl chain‐functionalized CFE. The noncovalent cholesterol‐alkyl chain interactions effectively immobilize aptamers onto the CFE surface, allowing the generation of a highly selective system for probing neurochemical dynamics in living systems and opening up a vast array of new opportunities for designing in vivo sensors for exploring brain chemistry.