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Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells
Author(s) -
Robson Jonathan A.,
Kubánková Markéta,
Bond Tamzin,
Hendley Rian A.,
White Andrew J. P.,
Kuimova Marina K.,
WiltonEly James D. E. T.
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202008224
Subject(s) - fluorophore , carbon monoxide , bodipy , viscosity , fluorescence , chemistry , microscopy , fluorescence microscope , fluorescence lifetime imaging microscopy , biophysics , chemical engineering , materials science , organic chemistry , catalysis , optics , biology , physics , composite material , engineering
A new family of robust, non‐toxic, water‐compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity‐sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.