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Morphometric Cell Classification for Single‐Cell MALDI‐Mass Spectrometry Imaging
Author(s) -
Ščupáková Klára,
Dewez Frédéric,
Walch Axel K.,
Heeren Ron M. A.,
Balluff Benjamin
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202007315
Subject(s) - mass spectrometry imaging , maldi imaging , mass spectrometry , characterization (materials science) , molecular imaging , computational biology , chemistry , biology , nanotechnology , matrix assisted laser desorption/ionization , materials science , chromatography , genetics , organic chemistry , adsorption , desorption , in vivo
The large‐scale and label‐free molecular characterization of single cells in their natural tissue habitat remains a major challenge in molecular biology. We present a method that integrates morphometric image analysis to delineate and classify individual cells with their single‐cell‐specific molecular profiles. This approach provides a new means to study spatial biological processes such as cancer field effects and the relationship between morphometric and molecular features.