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Reconfigurable T‐junction DNA Origami
Author(s) -
Young Katherine G.,
Najafi Behnam,
Sant William M.,
Contera Sonia,
Louis Ard A.,
Doye Jonathan P. K.,
Turberfield Andrew J.,
Bath Jonathan
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202006281
Subject(s) - dna origami , nanotechnology , dna nanotechnology , holliday junction , dna , set (abstract data type) , computer science , topology (electrical circuits) , biological system , chemistry , materials science , nanostructure , engineering , biology , homologous recombination , biochemistry , electrical engineering , programming language
DNA self‐assembly allows the construction of nanometre‐scale structures and devices. Structures with thousands of unique components are routinely assembled in good yield. Experimental progress has been rapid, based largely on empirical design rules. Herein, we demonstrate a DNA origami technique designed as a model system with which to explore the mechanism of assembly. The origami fold is controlled through single‐stranded loops embedded in a double‐stranded DNA template and is programmed by a set of double‐stranded linkers that specify pairwise interactions between loop sequences. Assembly is via T‐junctions formed by hybridization of single‐stranded overhangs on the linkers with the loops. The sequence of loops on the template and the set of interaction rules embodied in the linkers can be reconfigured with ease. We show that a set of just two interaction rules can be used to assemble simple T‐junction origami motifs and that assembly can be performed at room temperature.