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DNA Nanolithography Enables a Highly Ordered Recognition Interface in a Microfluidic Chip for the Efficient Capture and Release of Circulating Tumor Cells
Author(s) -
Zhang Jialu,
Lin Bingqian,
Wu Lingling,
Huang Mengjiao,
Li Xingrui,
Zhang Huimin,
Song Jia,
Wang Wei,
Zhao Gang,
Song Yanling,
Yang Chaoyong
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202005974
Subject(s) - aptamer , microfluidics , nanolithography , nanotechnology , fabrication , materials science , microfluidic chip , nanoscopic scale , cleave , chip , nano , molecular biophysics , lab on a chip , dna , chemistry , computer science , telecommunications , biochemistry , genetics , alternative medicine , pathology , composite material , biology , medicine
Microfluidic chips with nano‐scale structures have shown great potential, but the fabrication and cost issues restrict their application. Herein, we propose a conceptually new “DNA nanolithography in a microfluidic chip” by using sub‐10 nm three‐dimensional DNA structures (TDNs) as frameworks with a pendant aptamer at the top vertex (ApTDN‐Chip). The nano‐scale framework ensures that the aptamer is in a highly ordered upright orientation, avoiding the undesired orientation or crowding effects caused by conventional microfluidic interface fabrication processes. Compared with a monovalent aptamer modified chip, the capture efficiency of ApTDN‐Chip was enhanced nearly 60 % due to the highly precise dimension and rigid framework of TDNs. In addition, the scaffolds make DNase I more accessible to the aptamer with up to 83 % release efficiency and 91 % cell viability, which is fully compatible with downstream molecular analysis. Overall, this strategy provides a novel perspective on engineering nano‐scaffolds to achieve a more ordered nano‐topography of microfluidic chips.