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A Multi‐responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super‐resolution Imaging
Author(s) -
Yang Guanqing,
Liu Zhengjie,
Zhang Ruilong,
Tian Xiaohe,
Chen Juan,
Han Guangmei,
Liu Bianhua,
Han Xinya,
Fu Yao,
Hu Zhangjun,
Zhang Zhongping
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202005959
Subject(s) - sted microscopy , nucleic acid , nucleoprotein , mitochondrion , biophysics , fluorescence , chemistry , organelle , mitochondrial dna , resolution (logic) , microbiology and biotechnology , biology , biochemistry , dna , superresolution , physics , quantum mechanics , artificial intelligence , computer science , gene , image (mathematics)
Understanding the biomolecular interactions in a specific organelle has been a long‐standing challenge because it requires super‐resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super‐resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H 2 O 2 ), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H 2 O 2 level leads to disassociation of nucleic acid–protein complexes.