Premium
Harnessing Effective Molarity to Design an Electrochemical DNA‐based Platform for Clinically Relevant Antibody Detection
Author(s) -
Rossetti Marianna,
Brannetti Simone,
Mocenigo Marco,
Marini Bruna,
Ippodrino Rudy,
Porchetta Alessandro
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202005124
Subject(s) - modular design , molar concentration , detection limit , antibody , monoclonal antibody , dna , immunotherapy , immunoassay , nanotechnology , computational biology , computer science , chemistry , combinatorial chemistry , materials science , immunology , biology , chromatography , biochemistry , immune system , organic chemistry , operating system
Easy‐to‐use platforms for rapid antibody detection are likely to improve molecular diagnostics and immunotherapy monitoring. However, current technologies require multi‐step, time‐consuming procedures that limit their applicability in these fields. Herein, we demonstrate effective molarity‐driven electrochemical DNA‐based detection of target antibodies. We show a highly selective, signal‐on DNA‐based sensor that takes advantage of antibody‐binding‐induced increase of local concentration to detect clinically relevant antibodies in blood serum. The sensing platform is modular, rapid, and versatile and allows the detection of both IgG and IgE antibodies. We also demonstrate the possible use of this strategy for the monitoring of therapeutic monoclonal antibodies in body fluids. Our approach highlights the potential of harnessing effective molarity for the design of electrochemical sensing strategies.