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Monitoring H 2 O 2 on the Surface of Single Cells with Liquid Crystal Elastomer Microspheres
Author(s) -
Li Weiwei,
Khan Mashooq,
Lin Ling,
Zhang Qiang,
Feng Shuo,
Wu Zengnan,
Lin JinMing
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202004326
Subject(s) - molecule , chemistry , membrane , liquid crystal , microsphere , elastomer , in situ , biophysics , nanotechnology , chemical engineering , materials science , organic chemistry , biochemistry , engineering , biology , optoelectronics
Abstract Live‐imaging of signaling molecules released from living cells is a fundamental challenge in life sciences. Herein, we synthesized liquid crystal elastomer microspheres functionalized with horse‐radish peroxidase (LCEM‐HRP), which can be immobilized directly on the cell membrane to monitor real‐time release of H 2 O 2 at the single‐cell level. LCEM‐HRP could report H 2 O 2 through a concentric‐to‐radial (C‐R) transfiguration, which is due to the deprotonation of LCEM‐HRP and the break of inter or intra‐chain hydrogen bonding in LCEM‐HRP caused by HRP‐catalyzed reduction of H 2 O 2 . The level of transfiguration of LCEM‐HRP revealed the different amounts of H 2 O 2 released from cells. The estimated detection sensitivity was ≈2.2×10 −7 μ m for 10 min of detection time. The cell lines and cell–cell heterogeneity was explored from different configurations. LCEM‐HRP presents a new approach for in situ real‐time imaging of H 2 O 2 release from living cells and can be the basis for seeking more advanced chemical probes for imaging of various signaling molecules in the cellular microenvironment.