Premium
Catalytically Active Hollow Fiber Membranes with Enzyme‐Embedded Metal–Organic Framework Coating
Author(s) -
Bell Daniel Josef,
Wiese Monika,
Schönberger Ariel Augusto,
Wessling Matthias
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202003287
Subject(s) - glucose oxidase , membrane , chemical engineering , coating , chemistry , permeation , immobilized enzyme , fiber , microfiltration , materials science , chromatography , enzyme , organic chemistry , biochemistry , engineering
Metal–organic frameworks (MOFs) are suitable enzyme immobilization matrices. Reported here is the in situ biomineralization of glucose oxidase (GOD) into MOF crystals (ZIF‐8) by interfacial crystallization. This method is effective for the selective coating of porous polyethersulfone microfiltration hollow fibers on the shell side in a straightforward one‐step process. MOF layers with a thickness of 8 μm were synthesized, and fluorescence microscopy and a colorimetric protein assay revealed the successful inclusion of GOD into the ZIF‐8 layer with an enzyme concentration of 29±3 μg cm −2 . Enzymatic activity tests revealed that 50 % of the enzyme activity is preserved. Continuous enzymatic reactions, by the permeation of β‐ d ‐glucose through the GOD@ZIF‐8 membranes, showed a 50 % increased activity compared to batch experiments, emphasizing the importance of the convective transport of educts and products to and from the enzymatic active centers.