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Micro‐Lensed Fiber Laser Desorption Mass Spectrometry Imaging Reveals Subcellular Distribution of Drugs within Single Cells
Author(s) -
Meng Yifan,
Cheng Xiaoling,
Wang Tongtong,
Hang Wei,
Li Xiaoping,
Nie Wan,
Liu Rong,
Lin Zheng,
Hang Le,
Yin Zhibin,
Zhang Baolin,
Yan Xiaomei
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202002151
Subject(s) - mass spectrometry imaging , mass spectrometry , desorption , laser , nanoscopic scale , nanotechnology , chemistry , materials science , chromatography , adsorption , optics , physics , organic chemistry
The visualization of temporal and spatial changes in the intracellular environment has great significance for chemistry and bioscience research. Mass spectrometry imaging (MSI) plays an important role because of its unique advantages, such as being label‐free and high throughput, yet it is a challenge for laser‐based techniques due to limited lateral resolution. Here, we develop a simple, reliable, and economic nanoscale MSI approach by introducing desorption laser with a micro‐lensed fiber. Using this integrated platform, we achieved 300 nm resolution MSI and successfully visualized the distribution of various small‐molecule drugs in subcellular locations. Exhaustive dynamic processes of anticancer drugs, including releasing from nanoparticle carriers entering nucleus of cells, can be readily acquired on an organelle scale. Considering the simplicity and universality of this nanoscale desorption device, it could be easily adapted to most of laser‐based mass spectrometry applications.