Premium
Machine‐Driven Chemoenzymatic Synthesis of Glycopeptide
Author(s) -
Zhang Jiabin,
Liu Ding,
Saikam Varma,
Gadi Madhusudhan R.,
Gibbons Christopher,
Fu Xuan,
Song Heliang,
Yu Jin,
Kondengaden Shukkoor M.,
Wang Peng G.,
Wen Liuqing
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.202001124
Subject(s) - glycan , glycopeptide , oligonucleotide , chemistry , combinatorial chemistry , peptide synthesis , amine gas treating , peptide , solid phase synthesis , organic chemistry , biochemistry , glycoprotein , antibiotics , dna
Historically, researchers have put considerable effort into developing automation systems to prepare natural biopolymers such as peptides and oligonucleotides. The availability of such mature systems has significantly advanced the development of natural science. Over the past twenty years, breakthroughs in automated synthesis of oligosaccharides have also been achieved. A machine‐driven platform for glycopeptide synthesis by a reconstructed peptide synthesizer is described. The designed platform is based on the use of an amine‐functionalized silica resin to facilitate the chemical synthesis of peptides in organic solvent as well as the enzymatic synthesis of glycan epitopes in the aqueous phase in a single reaction vessel. Both syntheses were performed by a peptide synthesizer in a semiautomated manner.