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Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers
Author(s) -
Steinmetzger Christian,
Bäuerlein Carmen,
Höbartner Claudia
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201916707
Subject(s) - aptamer , förster resonance energy transfer , nucleobase , supramolecular chemistry , rna , chemistry , ligand (biochemistry) , fluorescence , nucleotide , combinatorial chemistry , biophysics , dna , crystallography , biochemistry , biology , microbiology and biotechnology , gene , crystal structure , receptor , physics , quantum mechanics
RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence‐based strategies reveal information on structure and dynamics of RNA aptamers. Herein, we report the incorporation of the universal emissive nucleobase analog 4‐cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to the bound ligands DMHBI + or DMHBO + . The photophysical properties of the new nucleobase–ligand‐FRET pair revealed structural restraints for the overall RNA aptamer organization and identified nucleotide positions suitable for FRET‐based readout of ligand binding. This strategy is generally suitable for binding‐site mapping and may also be applied for responsive aptamer devices.