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Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging
Author(s) -
Ko Jina,
Oh Juhyun,
Ahmed Maaz S.,
Carlson Jonathan C. T.,
Weissleder Ralph
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201915153
Subject(s) - tetrazine , fluorescence , bioorthogonal chemistry , quenching (fluorescence) , single cell analysis , kinetics , chemistry , cycling , biophysics , multiplex , fluorescence lifetime imaging microscopy , nanotechnology , materials science , cell , combinatorial chemistry , bioinformatics , biology , click chemistry , biochemistry , physics , history , organic chemistry , archaeology , quantum mechanics
Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra‐fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/ trans ‐cyclooctene (TCO)‐mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster ( t 1/2 <1 s) than predicted. This allowed multi‐cycle staining and immune cell profiling within an hour, leveraging the accelerated kinetics to open new diagnostic possibilities for rapid cellular analyses.