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Chimeric LuxR Transcription Factors Rewire Natural Product Regulation
Author(s) -
Mukherji Ruchira,
Zhang Shuaibing,
Chowdhury Somak,
Stallforth Pierre
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201914449
Subject(s) - quorum sensing , biology , gene , activator (genetics) , bacterial transcription , transcription factor , natural product , gene cluster , computational biology , genetics , promoter , microbiology and biotechnology , gene expression , biochemistry , virulence
LuxR‐type transcriptional activator proteins frequently regulate the expression of biosynthetic gene clusters (BGCs). With only a fraction of bacterial BGCs being expressed under standard culturing conditions, modulation of LuxRs would provide a powerful approach to activate silent clusters. We show that by exploiting the modular nature of LuxR proteins, it is possible to construct functional chimeric LuxRs, which enables both the rewiring of quorum sensing systems and the activation of silent BGCs. Importantly, our strategy allowed us to identify the novel natural product pseudomonol from a bacterium of the genus Pseudomonas .