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An Inorganic Biopolymer Polyphosphate Controls Positively Charged Protein Phase Transitions
Author(s) -
Wang Xin,
Shi Chenke,
Mo Jianbin,
Xu Yun,
Wei Wei,
Zhao Jing
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201913833
Subject(s) - polyphosphate , biopolymer , coacervate , citrobacter freundii , chemistry , biophysics , intracellular , phase (matter) , biochemistry , phosphate , biology , polymer , organic chemistry , escherichia coli , klebsiella pneumoniae , gene
Polyphosphate (PolyP) is one of the most compact inorganic polyanionic biopolymers that participates in various physiological processes. However, the mechanism of the interaction between polyP and proteins remains poorly understood. Herein, we report that polyP can interact with positively charged green fluorescent protein, +36GFP, resulting in liquid–liquid phase separation (LLPS) by intermolecular electrostatic interactions in cells. Upon nutrient deprivation, genetically engineered Citrobacter freundii accumulates intracellular polyP at a rate of 210 μ m min −1 , resulting in the compartmentation of +36GFP at the cell poles within 1 h. Medium chain‐length polyP (60‐mer) could induce the formation of +36GFP coacervates in vitro at a protein concentration as low as 200 n m , which is of the same magnitude as native proteins. In contrast, shorter polyP (14‐mer) could not induce LLPS under the same conditions. This may offer a general approach to manipulate protein–protein interactions through LLPS.