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Untargeted Tumor Metabolomics with Liquid Chromatography–Surface‐Enhanced Raman Spectroscopy
Author(s) -
Xiao Lifu,
Wang Chuanqi,
Dai Chen,
Littlepage Laurie E.,
Li Jun,
Schultz Zachary D.
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201912387
Subject(s) - metabolomics , metabolome , metabolite , biomolecule , chemistry , surface enhanced raman spectroscopy , chromatography , liquid chromatography–mass spectrometry , bioanalysis , raman scattering , mass spectrometry , raman spectroscopy , analyte , biochemistry , physics , optics
Abstract Metabolomics is a powerful systems biology approach that monitors changes in biomolecule concentrations to diagnose and monitor health and disease. However, leading metabolomics technologies, such as NMR and mass spectrometry (MS), access only a small portion of the metabolome. Now an approach is presented that uses the high sensitivity and chemical specificity of surface‐enhanced Raman scattering (SERS) for online detection of metabolites from tumor lysates following liquid chromatography (LC). The results demonstrate that this LC‐SERS approach has metabolite detection capabilities comparable to the state‐of‐art LC‐MS but suggest a selectivity for the detection of a different subset of metabolites. Analysis of replicate LC‐SERS experiments exhibit reproducible metabolite patterns that can be converted into barcodes, which can differentiate different tumor models. Our work demonstrates the potential of LC‐SERS technology for metabolomics‐based diagnosis and treatment of cancer.