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A Smart, Autocatalytic, DNAzyme Biocircuit for in Vivo, Amplified, MicroRNA Imaging
Author(s) -
Wei Jie,
Wang Huimin,
Wu Qiong,
Gong Xue,
Ma Kang,
Liu Xiaoqing,
Wang Fuan
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201911712
Subject(s) - deoxyribozyme , chemistry , in vivo , autocatalysis , microrna , preclinical imaging , nanotechnology , biophysics , dna , biochemistry , biology , materials science , genetics , gene , catalysis
Abstract DNAzymes have been recognized as promising transducing agents for visualizing endogenous biomarkers, but their inefficient intracellular delivery and limited amplification capacity (including insufficient cofactor supply) preclude their extensive biological application. Herein, an autocatalytic DNAzyme (ACD) biocircuit is constructed for amplified microRNA imaging in vivo based on a hybridization chain reaction (HCR) and DNAzyme biocatalysis, sustained by a honeycomb MnO 2 nanosponge (hMNS). The hMNS not only delivers DNA probes, but also supplies Mn 2+ as a DNAzyme cofactor and magnetic resonance imaging (MRI) agent. Through the subsequent cross‐activation of HCR and DNAzyme amplicons, the ACD amplifies the limited signal resulting from miRNA recognition. The hMNS/ACD system was used to image microRNA in vivo, thus demonstrating its great promise in cancer diagnosis.