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On‐Demand Electrochemical Epoxidation in Nano‐Electrospray Ionization Mass Spectrometry to Locate Carbon–Carbon Double Bonds
Author(s) -
Tang Shuli,
Cheng Heyong,
Yan Xin
Publication year - 2020
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201911070
Subject(s) - double bond , electrospray ionization , chemistry , mass spectrometry , electrochemistry , tandem mass spectrometry , electrospray , carbon nanotube , nanotechnology , chromatography , organic chemistry , materials science , electrode
Reported here is the first on‐demand electrochemical epoxidation incorporated into the standard nano‐electrospray ionization mass spectrometry (nanoESI‐MS) workflow for double‐bond identification. The capability lies in a novel tunable electro‐epoxidation of double bonds, where onset of the reaction can be controlled by simply tuning the spray voltage. On‐demand formation of mono‐/multiple epoxides is achieved at different voltages. The electro‐epoxidized products are then fragmented by tandem MS to generate diagnostic ions, indicating the double bond position(s). The process is completed within seconds, holding great potential for high‐throughput analysis. The rapid switch‐on/off electro‐epoxidation of a single sample, the low sample consumption, the demonstrated applicability to complex lipids containing multiple double bonds, and the advantage of not requiring extra apparatus make this method attractive for use in lipid‐related biological studies.