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Metal‐Chelated Polymer Nanodiscs for NMR Studies
Author(s) -
Hardin Nathaniel Z.,
Kocman Vojč,
Di Mauro Giacomo M.,
Ravula Thirupathi,
Ramamoorthy Ayyalusamy
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201910118
Subject(s) - biomolecule , nanodisc , polymer , chemistry , chelation , metal ions in aqueous solution , paramagnetism , metal , lipid bilayer , inorganic chemistry , organic chemistry , membrane , biochemistry , physics , quantum mechanics
Paramagnetic relaxation enhancement (PRE) is commonly used to speed up spin lattice relaxation time (T 1 ) for rapid data acquisition in NMR structural studies. Consequently, there is significant interest in novel paramagnetic labels for enhanced NMR studies on biomolecules. Herein, we report the synthesis and characterization of a modified poly(styrene‐co‐maleic acid) polymer which forms nanodiscs while showing the ability to chelate metal ions. Cu 2+ ‐chelated nanodiscs are demonstrated to reduce the T 1 of protons for both polymer and lipid‐nanodisc components. The chelated nanodiscs also decrease the proton T 1 values for a water‐soluble DNA G‐quadruplex. These results suggest that polymer nanodiscs functionalized with paramagnetic tags can be used to speed‐up data acquisition from lipid bilayer samples and also to provide structural information from water‐soluble biomolecules.