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Rational Design of pH‐Responsive DNA Motifs with General Sequence Compatibility
Author(s) -
Fu Wenhao,
Tang Linlin,
Wei Gaohui,
Fang Liang,
Zeng Jie,
Zhan Renjie,
Liu Xuemei,
Zuo Hua,
Huang Cheng Zhi,
Mao Chengde
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201906972
Subject(s) - dna , molecular machine , nanotechnology , dna origami , rational design , dna nanotechnology , structural motif , drug delivery , control reconfiguration , chemistry , computational biology , biosensor , computer science , combinatorial chemistry , biophysics , materials science , biology , biochemistry , embedded system
Reconfigurable molecular events are key to molecular machines. In response to external cues, molecular machines rearrange/change their structures to perform certain functions. Such machines exist in nature, for example cell surface receptors, and have been artificially engineered. To be able to build sophisticated and efficient molecular machines for an increasing range of applications, constant efforts have been devoted to developing new mechanisms of controllable structural reconfiguration. Herein, we report a general design principle for pH‐responsive DNA motifs for general DNA sequences (not limited to triplex or i‐motif forming sequences). We have thoroughly characterized such DNA motifs by polyacrylamide gel electrophoresis (PAGE) and fluorescence spectroscopy and demonstrated their applications in dynamic DNA nanotechnology. We expect that it will greatly facilitate the development of DNA nanomachines, biosensing/bioimaging, drug delivery, etc.