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Inhibition of Pathogen Adhesion by Bacterial Outer Membrane‐Coated Nanoparticles
Author(s) -
Zhang Yue,
Chen Yijie,
Lo Christopher,
Zhuang Jia,
Angsantikul Pavimol,
Zhang Qiangzhe,
Wei Xiaoli,
Zhou Zhidong,
Obonyo Marygorret,
Fang Ronnie H.,
Gao Weiwei,
Zhang Liangfang
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201906280
Subject(s) - bacterial adhesin , adhesion , bacterial outer membrane , chemistry , bacteria , membrane , nanomedicine , helicobacter pylori , biophysics , microbiology and biotechnology , cell adhesion , biofilm , nanoparticle , nanotechnology , biochemistry , escherichia coli , biology , materials science , gene , genetics , organic chemistry
Abstract Anti‐adhesion therapies interfere with the bacterial adhesion to the host and thus avoid direct disruption of bacterial cycles for killing, which may alleviate resistance development. Herein, an anti‐adhesion nanomedicine platform is made by wrapping synthetic polymeric cores with bacterial outer membranes. The resulting bacterium‐mimicking nanoparticles (denoted “OM‐NPs”) compete with source bacteria for binding to the host. The “top‐down” fabrication of OM‐NPs avoids the identification of the adhesins and bypasses the design of agonists targeting these adhesins. In this study, OM‐NPs are made with the membrane of Helicobacter pylori and shown to bind with gastric epithelial cells (AGS cells). Treatment of AGS cells with OM‐NPs reduces H. pylori adhesion and such anti‐adhesion efficacy is dependent on OM‐NP concentration and its dosing sequence.