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Visualization of Intra‐neuronal Motor Protein Transport through Upconversion Microscopy
Author(s) -
Zeng Xiao,
Chen Shuo,
Weitemier Adam,
Han Sanyang,
Blasiak Agata,
Prasad Ankshita,
Zheng Kezhi,
Yi Zhigao,
Luo Baiwen,
Yang InHong,
Thakor Nitish,
Chai Chou,
Lim KahLeong,
McHugh Thomas J.,
All Angelo H.,
Liu Xiaogang
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201904208
Subject(s) - axoplasmic transport , motor protein , neuroscience , transport protein , biophysics , nanotechnology , computer science , chemistry , materials science , biology , microbiology and biotechnology , microtubule
Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real‐time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single‐particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra‐axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.