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Peptide‐Directed DNA‐Templated Protein Labelling for The Assembly of a Pseudo‐IgM
Author(s) -
Nielsen Thorbjørn B.,
Thomsen Rasmus P.,
Mortensen Michael R.,
Kjems Jørgen,
Nielsen Per Franklin,
Nielsen Thomas E.,
Kodal Anne Louise B.,
Cló Emiliano,
Gothelf Kurt V.
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201903134
Subject(s) - oligonucleotide , dna , chemistry , dna sequencing , peptide , biochemistry , microbiology and biotechnology , combinatorial chemistry , biology
The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein‐binding peptides can direct a DNA‐templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star‐like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo‐IgM antibody structure that was subsequently characterized by negative‐stain transmission electron microscopy (nsTEM) analysis.