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Enzymatic/Immunoassay Dual‐Biomarker Sensing Chip: Towards Decentralized Insulin/Glucose Detection
Author(s) -
Vargas Eva,
Teymourian Hazhir,
Tehrani Farshad,
Eksin Ece,
SánchezTirado Esther,
Warren Paul,
Erdem Arzum,
Dassau Eyal,
Wang Joseph
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201902664
Subject(s) - immunoassay , biosensor , glucose oxidase , chemistry , chromatography , bioassay , detection limit , biomarker , biochemistry , antibody , biology , genetics , immunology
Performing bioassay formats based on enzyme and antibody recognition reactions with a single detection chip remains an unmet challenge owing to the different requirements of such bioassays. Herein, we describe a dual‐marker biosensor chip, integrating enzyme and antibody‐based assays for simultaneous electrochemical measurements of insulin (I) and glucose (G). Simultaneous G/I sensing has been realized by addressing key fabrication and operational challenges associated with the different assay requirements and surface chemistry. The I immunosensor relies on a peroxidase‐labeled sandwich immunoassay, while G is monitored through reaction with glucose oxidase. The dual diabetes biomarker chip offers selective and reproducible detection of picomolar I and millimolar G concentrations in a single microliter sample droplet within less than 30 min, including direct measurements in whole blood and saliva samples. The resulting integrated enzymatic‐immunoassay biosensor chip opens a new realm in point‐of‐care multiplexed biomarker detection.