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Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria
Author(s) -
Kwon HawYoung,
Liu Xiao,
Choi Eun Gyeong,
Lee Jung Yeol,
Choi SoYoung,
Kim JunYoung,
Wang Lu,
Park SungJin,
Kim Beomsue,
Lee YongAn,
Kim JongJin,
Kang Nam Young,
Chang YoungTae
Publication year - 2019
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201902537
Subject(s) - bacteria , gram staining , gram , microbiology and biotechnology , fluorescence , gram positive bacteria , in vivo , gram negative bacteria , biology , chemistry , escherichia coli , biochemistry , gene , genetics , optics , physics
The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram‐positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram‐positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application.